Curriculum vitae
Personal Information
- Address: Via Adolfo Ferrata 17, Pavia 27100 (PV), Italy
- Date of Birth: 03/04/1994
- Nationality: Lebanese
- Email: aboualezz.monah@hsr.it
Professional Experience
October 2020 - Current
Postodoctoral researcher, San Raffaele Telethon Institute for Gene Therapy (TIGET)
- Bioinformatics Core
- Projects:
- Retrovirus-host interactions and innate immunity to gene transfer: Prof. Anna Kajaste-Rudnitski
- Epigenetic regulation and targeted genome editing: Prof. Angelo Lombardo
- Human hematopoietic development and disease modeling: Dr. Andrea Ditadi
September 2019 - Current
Software and Data Carpentry Instructor, The Carpentries
A certified instructor by The Carpentries organization, California - USA. Teaching foundational coding and data science skills to researchers in workshops worldwide.
Workshops:
- Rutgers University: Genomics Workshop Instructor: Project management/ Introduction to cloud computing, Jan 26 - 29, 2021
- Augusta University: Library Carpentry Instructor: Version control with Git/ The Unix Shell, Jan 26 - Feb 04, 2021
- NIH Network of the National Library of Medicine: Library Carpentry Instructor: SQL, Jan 07 - 08, 2021
- Genentech, A member of the Roche group: Software Carpentry instructor: Plotting and Programming in Python, Dec 14 - 17, 2020
- Auburn University: Library Carpentry instructor: The Unix shell and Bash scripting, Dec 07 - 11, 2020
- Stony Brook University: Software Carpentry instructor: Programming with R, Sep 12 - 13, 2020
- North Carolina Central University: Data Carpentry instructor: Introduction to R and data visualisation, Aug 12 - 14, 2020
- University of Stuttgart: Software carpentry instructor: Building programs with python, Nov 21 - 22, 2019
- University of Milano Bicocca: Data Carpentry instructor: Introduction to Python and data visualisation, Feb 14 - 15, 2019
October 2018 - Current
Bioinformatics Tutor, University of Pavia
- Bioinformatics Course of the Masters in Molecular Biology and Genetics
- Training and practical sessions in bioinformatics tools
October 2017 - September 2020
PhD internship, Institute of Molecular Genetics Luigi Luca Cavalli-Sforza, National Research Council
- Laboratory of Computational Biology
- Project: Characterization of long non-coding RNAs genomic and splicing features
- PI: Prof. Silvia Bione
July 2017 - October 2017
ERASMUS+ Traineeship, Institute of Plant Genetics, Polish Academy of Sciences
- Laboratory of Plant Genetics
- Project: Bioinformatics characterization and identification of miRNAs in woody species
- PI: Dr. Jorge A. Pinto Paiva
November 2015 - July 2017
Masters Internship, University of Pavia - Department of Biology and Biotechnology L.Spallanzani
- Laboratory of Cell and Neurobiology
- Influence of divalent cations on DNA methylation and the modulation of chromatin structure in an in situ ultrastructural study
- PI: Prof. Marco Biggiogera
July 2009 - July 2015
Laboratory Assistant, Lebanese Agriculture Research Institution (LARI)
- Department of Crop Production
- Project: Quality analysis on commercial wheat for classification and marketing
- PI: Eng. Chafic Stephane
- Department of Plant Breeding and Improvement
- Project: Identification of new promising varieties of Triticum aestivum, Hordeum vulgare, and Lens culinaris with high productivity, good grain quality and good adaptation to different climatic conditions.
- PI: Eng. Rabih Kabalan
Education
2017 - 2020
University of Pavia
PhD in Genetics, Molecular and Cellular Biology
2015 - 2017
University of Pavia
Masters Degree in Molecular Biology and Genetics
2012 - 2015
Lebanese University
Bachelor Degree in Earth and Life Sciences
Skills
Bioinformatics Skills
- Proficient knowledge of Windows 7/8/10, MacOS and Linux operative systems
- Proficient knowledge of Linux environment (Ubuntu)
- Proficient knowledge of bash and shell scripting
- Proficient knowledge of AWS (Amazon Web Services) clusters (S3 and EC2 instances) and Cloud Services
- Proficient knowledge in machine learning approaches
- Proficient knowledge of R programming language
- Proficient knowledge in Python programming language
- Basic knowledge in Perl programming language
- Basic knowledge in SQL
- Proficient knowledge in version control and source code management tools
- Proficient knowledge in docker containers
- Proficient in Next Generation Sequencing analysis tool and devoloping data analysis pipelines
- Proficient knowledge in data mining and data visualisation tools
- Proficient in utilizing genomic databases
- Proficient in utilizing Galaxy web-interface
- Proficient knowledge in Microsoft office suite
- Image Analysis Software: Adobe Photoshop, ImageJ, Cell F
Wet Laboratory Skills
- Proficient in Electron Microscopy Techniques: fixation, pre-embedding, dehydration, inclusion, staining (uranyl acetate, terbium, osmium amine), ultramicrotome
- Proficient in Electron Microscopy Operations and Handling (ZEISS EM 900)
- Proficient in Fluorescence Microscopy Operations
- Proficient in Light Microscopy: bright field, dark field, phase contrast, DIC
- Proficient in Electron Spectroscopic Imaging Operations
- Proficient in Immunocytochemistry and Immunofluorescence
- RNA, DNA and Plasmid Extraction
- Gene Cloning
- UV and Light Spectrophotometry
- Proficient in PCR and Primer Designing
- Western Blot
- Microbiology Laboratory Techniques: gram staining, bacterial cell culture, antibiotic specificity, and bacterial identification biochemical testing.
- Anatomy and Dissection of Vertebrates and Invertebrates
- Protein Extraction - Bradford Protein Assay
- ELISA Assay
- In-vitro Plant Cell Culture
Language Skills
- Mother-tongue Language: Arabic
- Proficient in English (B2) and Italian (B1) Languages
- Novice in French (A1) and Polish (A1) Languages
Awards
2019
Best Poster Prize, EMBL-EMBO Symposium: The Non-Coding Genome
2018
Fellowship Grant, AIRC Foundation
2018
Travel Grant, INTEROMICS
2017
Fellowship Grant, Besta Neurological Institute
2017
ERASMUS+ fellowship, University of Pavia
Publications
Journals
2020
Abou Alezz M, Celli L, Belotti G, Lisa A and Bione S. 2020. GC-AG Introns Features in Long Non-coding and Protein-Coding Genes
Suggest Their Role in Gene Expression Regulation. Front. Genet. 11:488. doi:10.3389/fgene.2020.00488
2020
Abou Alezz M, Salviati L, Nicastro A, Belotti G, Alfieri R, Lisa A, Bione S. 2020. A Preferential enrichment of GC donor splice sites in
wobble splicing. Under submission.
Software
2020
Abou Alezz M, Salviati L, Alfieri R, Bione S. 2020. GencoDymo: Data Extraction and Manipulation from the GENCODE Database.
R-package version 0.2.1. https://github.com/monahton/GencoDymo. doi:10.5281/zenodo.3605995
Oral Presentations and Abstracts
2020
Abou Alezz M, Celli L, Belotti G, Salviati L, Nicastro A, Alfieri R, Lisa A, Bione S. GC-AG introns features in long non-coding and
protein-coding genes suggest their role in gene expression regulation - 22nd EMBL PhD Symposium: The Roaring 20s - Online
conference
2020
Abou Alezz M, Celli L, Belotti G, Salviati L, Nicastro A, Alfieri R, Lisa A, Bione S. GC-AG introns features in long non-coding and
protein-coding genes suggest their role in gene expression regulation” - Cancer Science Institute of Singapore: 6th RNA Biology Symposium 2020 - Online
conference
2020
Abou Alezz M, Celli L, Belotti G, Salviati L, Nicastro A, Alfieri R, Lisa A, Bione S. GC-AG introns features in long non-coding and
protein-coding genes suggest their role in gene expression regulation” - 25th Annual meeting of the RNA Society - Online
conference
2020
Abou Alezz M, Celli L, Belotti G, Lisa A, Bione S. Unexpected features of GC-AG introns in long non-coding and protein-coding
genes suggest a new role as regulatory elements – Pavia 3rd Annual Joint Symposium – LIFE SCIENCE 2020 - Pavia, Italy
2019
Abou Alezz M, Celli L, Belotti G, Bione S. Characterization of the genomic and splicing features of long non-coding RNAs using
bioinformatics approaches - EMBL Symposia 2019: The Non-Coding Genome - Heidelberg, Germany
2018
Borowska M, Abou Alezz M, Jarząb M, Gomes C, Balestrazzi A, Paiva J. Preliminary overview on conserved miRNAs in Salix
purpurea L. - Integrative Plant Biology Conference, IPG PAS - Poznań, Poland
2018
Abou Alezz M, Celli L, Bione S. Computational approach towards genomic characterization of human long non-coding RNA -
EMBO Workshop 2018: From epigenome towards epitranscriptome in cell fate choice - Capri, Italy
2018
Masiello I, Siciliani S, Abou Alezz M, Bina V, Casali C, Stortiero M, Tollemeto V, Biggiogera M. High resolution dissection of
interphase chromatin. Is 5-methylcytosine the main character in chromatin condensation? – 22nd International Chromosome
Conference - Prague, Czech Republic
2017
Biggiogera M, Abou Alezz M, Bina V, Stortiero M, Tollemeto V, Masiello I. Heterogeneity of perichromatin fibril-associated factors -
25th Wilhelm Bernhard Workshop on the Cell Nucleus – Nizhny Novgorod, Russia
2016
Biggiogera M, Tollemeto V, Bina V, Abou Alezz M, Masiello I. Transcription time window applied to RNA modifications in HeLa cells
– Nuclear structure and dynamics through the microscopes symposium - Pavia, Italy
Last updated: 11/02/2021