Calculate GC Content of Genomic Features — calculate_gc_content • GencoDymo2

Computes the GC content percentage for exons or introns using genome sequence data from a BSgenome object.

Usage

calculate_gc_content(input, genome, verbose = TRUE)

Arguments

input: A data frame containing genomic features (exons/introns) with seqnames, start or intron_start, end or intron_end, and strand columns.
genome: A BSgenome object representing the reference genome (e.g., BSgenome.Hsapiens.UCSC.hg38).
verbose: A logical indicating whether to print progress messages. Defaults to TRUE.

Value

The input data frame with an additional gc_content column containing GC percentages for each feature.

Examples

if (FALSE) { # \dontrun{
if (requireNamespace("BSgenome.Hsapiens.UCSC.hg38", quietly = TRUE)) {
  genome <- BSgenome.Hsapiens.UCSC.hg38::BSgenome.Hsapiens.UCSC.hg38
  file_v1 <- system.file("extdata", "gencode.v1.example.gtf.gz", package = "GencoDymo2")
  gtf_v1 <- load_file(file_v1)
  gtf_with_gc <- calculate_gc_content(gtf_v1, genome = genome, verbose = FALSE)
}
} # }